Modification of Turnip yellow mosaic virus coat protein and its effect on virion assembly

In vitro synthesis of turnip yellow mosaic virus coat protein in a wheat germ cell-free system.

Turnip Yellow Mosaic Virus RNA directs the synthesis in vitro of its coat protein in a wheat germ cell-free extract. Optimum conditions for synthesis have been defined, and the effect of spermine on specifically enhancing coat protein formation has been examined. Identity between the in vitro synthesized coat protein and authentic coat protein of Turnip Yellow Mosaic Virus was established by an...

Translation of turnip yellow mosaic virus RNA in vitro: a closed and an open coat protein cistron.

Sucrose gradient centrifugation of heat-denatured RNA of turnip yellow mosaic virus permitted the isolation of five RNA classes with molecular weights ranging from 2.0 to 0.25 X 10(6). The infectivity was shown to be confined to an RNA molecule of molecular weight 2.0 X 10(6). No significant increase in infectivity was obtained by combination of the latter RNA with the RNA classes of smaller si...

Partial Epitope Mapping of Alfalfa mosaic Virus and the Effect of Coat Protein Gene Mutation on Aphid Transmission

Polyvalent display of RGD motifs on turnip yellow mosaic virus for enhanced stem cell adhesion and spreading.

Turnip yellow mosaic virus (TYMV) is a stable 28 nm icosahedral plant virus that can be isolated in gram quantities. In order to study the polyvalent effect of Arg-Gly-Asp (RGD) clustering on the response of bone marrow stem cells (BMSCs), an RGD motif was genetically displayed on the coat protein of the TYMV capsid. Composite films composed of either wild-type TYMV or TYMV-RGD44, in combinatio...

Specificity of TMV RNA encapsidation: in vitro coating of heterologous RNA by TMV protein.

Tobacco mosaic virus (TMV) RN.4 and TMV coat protein were reconstitut,ed in the presence of an excess of host RNA or turnip yellow mosaic virus (TYMV) RNA. TMV protein and its homologous RNA recognized each other specifically in the presence of an excess of host RNA, but when TMV RNA and TYMV RNA were mixed with protein, both RNAs were encapsidated. Indeed, at pH 6, TYMV RNA was recognized by T...